Methods and Submission Guide
Analytical methods, standards reference, and sample submission instructions for every panel.
All COAs include full methodology documentation and instrument data.
Methods and Specifications
Every panel uses validated analytical methods with full instrument data, chromatograms, and methodology documentation in the COA.
Chromatographic separation quantifies target peptide vs impurities, deletion sequences, and synthesis by-products. Basic ($160) or expanded with aggregate analysis ($200).
Mass spectrometry confirms peptide identity against reference standards. Verifies you have semaglutide, tirzepatide, BPC-157 — not a substitute. Included in expanded purity ($200).
Quantifies net peptide content adjusted for moisture, salts, and counter-ions. Confirms vial fill matches stated dose. Standalone ($200) or combined with purity ($350–$400).
Screens for lead, arsenic, cadmium, mercury, and other elemental impurities. ICP-MS only ($200) or full metals and elementals by ICP-MS and ICP-OES ($250).
LAL assay detects and quantifies gram-negative bacterial endotoxins. Required for all injectable peptides. Endotoxin method suitability available ($675) for validated repeat testing.
Standard USP 71 sterility with 14-day incubation ($175) or rapid Celsis method returning results in 5–7 days ($250). Method suitability required before first test ($1,800).
Additional Testing Options
Volatile Organic Compounds
GC-MS screening for residual solvents and VOCs from peptide synthesis. Common contaminants include TFA and acetic acid from HPLC purification steps ($200 each).
Container Closure Integrity
Verifies vial seal integrity for injectable products. Critical for multi-dose vials and lyophilized peptides stored long-term ($75).
Antimicrobial Effectiveness
USP 51 testing for preserved multi-dose formulations including bacteriostatic water preparations and multi-use injectable vials ($500).
pH Testing
Confirms formulation pH is within specification for injectable and topical preparations. Required for many 503A/503B release programs. Available standalone ($50) or added to any panel.
How to Submit a Sample
Contact us before shipping to confirm your panel and receive packaging instructions. Samples submitted without prior contact or without chain of custody documentation will be held pending follow-up.
Contact Us First
Confirm your panel selection and receive packaging instructions before shipping.
Package Your Sample
Most panels require 10–50mg lyophilized or liquid. Use the packaging guide we provide.
Include Chain of Custody
Complete and include the COC form with your shipment. Samples without COC will be held.
Ship Overnight
We accept overnight shipments from anywhere in the United States.
Sample Requirements by Panel
Chemical panels (HPLC, LC-MS, potency, heavy metals): 10–20mg lyophilized or equivalent liquid volume. Biological panels (endotoxin, sterility): 20–50mg or minimum 1mL liquid, endotoxin-free container. Full Injectable Panel: 50mg minimum to ensure sufficient material across all methods. For custom synthesized or novel sequences, contact us before submitting — minimum quantities may vary.
Regulatory Methods and Standards
Every Atumnus Labs method is performed against a named regulatory standard. Methodology documentation in every COA references the applicable standard by chapter and version.
Chromatography
Governs HPLC method parameters and system suitability criteria.
Bacterial Endotoxins
LAL assay — quantitative endotoxin detection for injectable preparations.
Sterility Tests
14-day incubation. Method suitability required per formulation.
Elemental Impurities
ICP-MS limits for lead, arsenic, cadmium, mercury, and other elements.
Antimicrobial Effectiveness
For preserved multi-dose formulations and bacteriostatic preparations.
Residual Solvents
GC-MS screening for synthesis residuals including TFA and acetic acid.
Ready to verify what's in the vial?
Submit a sample or request a quote. Our team responds within 24 hours.